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Thorpe TA Plant tissue culture, methods and applications in agriculture. Torres KC Tissue culture techniques for horticultural crops.

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Vasil IK Cell culture and somatic cell genetics of plants, vol 1: Laboratory procedures and their applications. Bailey LH Manual of cultivated plants. MacMillan Publishing, New York. Budavari S ed The Merck index: an encyclopedia of chemicals, drugs, and biologicals, 11th edn. Kluwer Academic, Dordrecht. Varsity Press, Athens, GA.


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Esau K Plant anatomy, 2nd edn. George EF, Sherrington PD Plant propagation by tissue culture: handbook and directory of commercial laboratories. Exegetics, Eversley, UK.

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Simmonds NW ed Evolution of crop plants. Longman, London. Sittig M, Noyes R Genetic engineering and biotechnology related firms worldwide directory, 8 th edn. Martinus Nijhoff, Dordrecht. Engelmann F Cryopreservation for the long-term conservation of tropical crops of commercial importance. Kyle L Plants from test tubes. In: Vasil I ed Cell culture and somatic cell genetics of plants, vol 8: Scale0up automation in plant tissue culture.

Phillips There are no affiliations available. Personalised recommendations. Cite chapter How to cite? ENW EndNote. Buy options. Schneider- 2 cells are adaptable to large-scale cultivation in stirred tank bioreactors andshow many of the properties of Sf9 cells. There is no CO, require- ment for buffering the medium. Origin-defective mutants of SV40 encoding wild-type T-antigen were used stably to transform monkey kidney cells.

Laboratory Facilities, Operation, and Management

The transformed COS cells, containing T antigen, are able to support the replicationof pure populations of recombinant Cells must not be allowed to reach confluence, in order to prevent syncitia formation. The NIH13T3 cell line is susceptible to sarcoma virus focus formation and leukaemia virus and is a valuable cell line for DNA transfection studies. Ithas been used for the expression of recombinant proteins, including hepatitis B surfaceantigen,phenylalanine hydroxylase, rat growth hormone, recombinantfibronectins, human class I1MHC antigens, human insulin receptor and insulin-like growth factor.

The cells are highly contact inhib- ited and are sensitive to serum batches. This is a widely studied cell line derived from a human cervix adenocarcinoma Gey et al. The cells are epithelial-like inmorphology and are susceptible to polio virus type 1and adenovirus type3. HeLa cells are used for theexpression of recombinant proteins, including mouse metallothionein 1gene, human CulZn superoxide dismutase and hepatitisB surface antigen. Theyhave been widely used as an in vitro model system because of the ease with which they can be cultivated but one drawback of this is that the cell line has been responsible forwidespread contamination of other cell lines Nelson-Rees et al.

Establishing a stem cell culture laboratory for clinical trials

The cell line isusedin transfection studies. The Vero monkey kidneycell line has been used for the industrial production of viral vaccines in preference to primary monkey kidney cells because of availability and the reduced risk of contamina- tion by endogenous viruses. Vero cells have been used for the production of the polio Sabin vaccine and shown to have identical vaccine characteristics to the primary monkey kidneycells Montagnon et al.


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Being a strictly anchorage- dependent cell line with fibroblast morphology, Vero cells have been adapted to culture in continuousperfusion systems, enabling high cell densities to beattained. Cell density at thetime of virus inoculation has been foundto influence the produc- tion of poliovirus -antigen, with continuous perfusion producing the highest cell densities Van der Meer et al.

A variant cell line adapted to grow in serum-free conditions is also available. It shouldbenotedthat the WorldHealthOrganization has sponsored the creation of a fully characterized cell bank for vaccine manufacture purposes. The cells are resis- 8-azaguanine and die in the presence of HAT medium.

S rictions are dependentupon cell. A number of monoclonalantibodies have been licensed orare undergoing clinical trialsfor therapeuticapplication, e. Campath-lraisedtothe lymphocyte C and used fortheprevention of graft versus hostdisease,antibodi cancerandseptic shock PMA, Important considerationsto reduce costs and downstreamprocessinginclude the necessity for high concentrations of antibody during production andhigh cell con- can be attainedusing perfusion systems Wang et al. A11of these systems are adaptable to large- scale production. An importantfactorinthe growth of cells in Some cultures may need to be started on mouse macrophage feeder layers.

These cells are used uction of viral vaccines, including rhinoviruses, Sabin poliovirus, strain, mumps, rubella, rabies, vaccinia and varicella. They show a sirnilarvirus susceptibilityto another huma iploid cell strain, WI see below. The cell line has a broad range of virus susceptibility and is used fortheproduction of human virus vaccines: rhinoviruses, measles, mumps, rubella, polio, rabiesand yellow fever. The cell line is used forthe production ofIFN-cx. Cultures have been scaled up for growth in large fermenters l Klein et al.

The Namalwa cell line is cited in a US andlor other patents andmust not be used to infringe patent claims. This is a Syrian hamster cell line derived from the kidneys of l-day-old hamsters. Smithet al.

Cell and tissue culture : laboratory procedures in biotechnology

Each genome contained a deletion of the? This practically eliminates the poten- tial transfer andlor recombination events that could lead totheproduction of helper virus wild-type replication competent virus by viral producers derived from these cell lines. These properties of the VC lines makethem particularly useful for in vivo g age analysis and development of human g E can be used to isolate clones that stably produce high titres lo6 cfu m1-l of recombinant retroviruses with amphotropic and ecotropic host ranges, respectively.

These cell lines are cited in a US patent and must not be used toinfringe claims. Stimulationbyhydro- 64 1 Finter NB umancells as a source of interferons for clinical use.

MilsteinC Preparation onalantibodies:strategiesand procedures. Cell Production of crudeinterferon. Eutterworth- Heinemann, Oxford. Science Virology KluwerAcademic, Dordrecht, The etherl lands. Kluwer Academic, Dordrecht, The Netherlands. Biologicals 18 2 : The large-scale culture of animal cells is nowa recognized approach for the manu- facture of biologicals. The regulatory aspects of such work are designedto ensure conformity and safety of such products and to a large degree dictate the way in which cultures are prepared, stored andquality controlled prior to and during the course of manufacture Center for esearch, This seed stock concept or seed lot systemisby no means new; however, its application in cell and tissue culture is unfortunately not universal.

The principles required for the reparation and handling of cell stocks in a manufacturing environment are equally relevant in the research labo- ratory to ensure correct identity, conformity and consistency of cell stocks. This section aims to give an overview of the general considerations in the production of master and working banks of animal cells. Hoechst stain and culture Absence of other adventitious agents e.

Full text of "Cell And Tissue Culture Laboratory Procedures In Biotechnology"

Larger ba prepared for stable continuous cell lines and this is a major advantageof this type of cell line in comparison with finite cell lines of limited replicative capacity. The number of cells per ampoule should be predetermined andvalidated for the ampoule of cells to regeneratea representative culture. An addi- requirement is thatthe cells shouldbe passaged beyondthe eve1 achieved at the end of each production run.

Strictly speaking, G to safety testing , but application ay be required as part of further procedures where the preparation of the cell products will be recorded andtestedto this standard. Job descriptions, curriculum vitae and training records for all scientific staff.